How to calibrate the EZO Complete-Dissolved Oxygen using the Atlas Desktop software


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The Atlas Scientific line of EZO Complete circuits makes building a custom monitoring system easy, but if you don’t calibrate your sensors, then what good are your readings; And can you trust them?

The accuracy of your readings is directly related to the quality of your calibration.

Calibration is not difficult, and a little bit of care goes a long way.

In this tutorial we’ll quickly go over how to properly calibrate the EZO-Complete Dissolved Oxygen, using the 100% free Atlas Desktop software. (click here to download now)

You will need the following:

Let’s get the obvious steps out of the way first!

To connect the EZO Complete-Dissolved Oxygen to your PC you’re going to need to use a Micro USB cable. Connect this now and run the Atlas Desktop Monitoring software.

Next, connect your Dissolved Oxygen probe to the EZO Complete-Dissolved Oxygen via the SMA connector.

Looking at the Atlas Desktop Monitoring software, you should see that a yellow rectangle is on the screen indicating that a Dissolved Oxygen circuit is connected, click on this yellow rectangle, to go to the Dissolved Oxygen screen.

Now that you are in the Dissolved Oxygen reading screen, click on the gears icon located on the bottom left corner of the screen. This will take you to the Dissolved Oxygen calibration screen.

Within this page you can calibrate your Dissolved Oxygen probe to the air; and to zero dissolved oxygen (calibration solution) which is optional. You can also clear the calibration from the memory in case you make a mistake or have recently attached a new probe to your EZO Complete-Dissolved Oxygen.

Calibrate to air

Before you place your probe into the calibration solution, you need to calibrate the probe to the air. To do this, simply leave the probe exposed to the air, until the readings become stable. Once the readings are stable, click on the “Calibrate to air” button. Once the probe is calibrated to the air, you should see readings of 9.09 – 9.1X mg/L.

Calibrating to zero dissolved oxygen

Again, this step is optional, you only need to perform this calibration if you require Dissolved Oxygen readings under 1 mg/L.

Open the pouch and pour out the solution into a small cup and place your probe into the solution. Make sure to stir the probe around to remove any trapped air (which could cause readings to go high) Wait a minute or two for the readings to stabilize on the screen. Once the readings have stabilized click the “Calibrate to Zero D.O.” button.

You could place your probe directly into the calibration pouch, but using a cup reduces the chances of spills.

The EZO Complete-Dissolved Oxygen is now completely calibrated, you are now ready to take readings. However, it’s good practice to rinse off your probe after calibration; And before placing it into your sample.

Stir it around a bit

Remember, when taking readings with your dissolved oxygen probe, it’s important to give it a small stir to remove any trapped bubbles underneath the probe. As these bubbles can cause your readings to go high.


Atlas Scientific recommends that calibration be done every 1 1.5 years.

Just in case

If you need just a bit more guidance on how to calibrate the Atlas Scientific EZO-Complete Dissolved Oxygen, you can follow along with the video below.

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