Dissolved oxygen probes work by measuring the amount of oxygen that diffuses across a permeable (or semi-permeable) membrane into a probe (sensor). Once oxygen is inside the sensor, a chemical reduction reaction occurs, producing an electrical signal. This signal is read by the DO probe and is displayed on a meter.
Dissolved oxygen (DO) comes from two main sources: the atmosphere (molecular oxygen) and photosynthesis, where it is a byproduct. Most aquatic organisms require DO to survive and it is important for chemical processes in aquatic environments.
Depending on the industry you work in, will depend on why you are testing DO and what DO probe is best for you. For example, if you are testing DO when brewing beer, the reason you test DO will be different from regularly testing water samples as a lab technician.
While there are many ways to test DO, if you are testing in the lab or the field, the easiest way to test DO in water is to use an electroanalytical or electrochemical dissolved oxygen sensor/probe, also known as amperometric or Clark-type sensors.
How Do Dissolved Oxygen Probes Work?
Most people are familiar with the titrimetric method to measure DO in water. Titrations use one liquid where the concentration is already determined (titrant), to identify the concentration of another (your sample), however, with advances in technology, precision equipment such as a DO probe offers precision and accuracy from measuring DO during basic laboratory work to industrial water quality monitoring.
A dissolved oxygen probe works off redox (oxidation-reduction) reactions, providing continuous and live measurements. As they have an applied voltage, most DO probes require a “warm-up time” before use which polarizes the electrodes before measuring the DO in water.
Once the DO probe is connected to the meter, the electrode sensor (DO probe) is dipped into the solution being tested. When the DO probe is connected to the meter, the voltage can be applied to the electrodes inside. The oxygen (O2) molecules reach the membrane, reaching the electrodes through the permeable/semi-permeable membrane via diffusion. A small electrical current flows between the electrodes (cathode and anode). The amount of current flowing between the electrodes is proportional to the O2 concentration of the solution. The amount of DO in the solution is measured by measuring the current flow using the calibrated meter.
Dissolved oxygen sensors do, however, have a disadvantage, while taking the DO measurement. It consumes an amount of O2 from the solution equal to the diffusion in the sensor. So, the amount of O2 near the DO probe is reduced, therefore, to obtain an accurate measurement if using a galvanic probe, the DO probe must be stirred in the solution.
Galvanic DO Probes/Sensors
Galvanic DO probes produce a voltage as O2 diffuses across the probe’s membrane. The thin semi-permeable membrane allows O2 to pass through, blocking anything else from passing.
As O2 passes through the membrane and electrolytic solution to the electrodes, it dissolves inside the probe cap that contains the buffered electrolyte which allows O2 to react with the cathode (platinum electrode), thus gaining an electron. It is this electron that is given to the O2 molecule that creates a voltage between the anode (silver chloride electrode) and cathode inside the DO probe.
Once the DO probe has detected a current, the meter attached can then convert the reading into a DO concentration.
Polarographic DO Probes/Sensors
Polarographic DO probes also contain a thin semi-permeable membrane, however, the voltage is applied between the anode and cathode in the DO probe. As O2 reaches the cathode, an electron is added to the O2 molecule, which creates a current. It is this voltage that drives the O2 reaction and determines the DO concentration.
At Atlas Scientific, we offer both Lab Grade and Industrial DO galvanic probes that can be used in a wide range of applications.
How To Use A Lab Grade DO Probe?
The Lab Grade DO probe contains a PTFE membrane, a cathode, and an anode soaked in an electrolyte. During use, the O2 molecules diffuse through the DO probe membrane at a continuous rate. Once the O2 molecules cross the membrane, they are reduced at the cathode, where a small voltage is produced. When no O2 molecules are present, the DO meter will read the voltage as 0 mV (millivolts). As the O2 molecules increase, so does the reading from the DO probe.
Once your probe has recorded the voltage, it can be easily read by a multimeter or an analogue digital converter.
The Lab Grade DO probe can be fully submerged indefinitely in fresh or saltwater up to the SMA connector.
How To Use An Industrial DO Probe?
The Industrial DO probe works the same as the Lab Grade probe, but it consists of a silicone membrane instead of a PTFE membrane. If you are using an Industrial DO probe, you can connect it to a DO Circuit and RTD Circuit via a Carrier Board, and for industrial processes, you can connect the DO probe to a DO transmitter.
The Industrial DO probe can also be fully submerged indefinitely in fresh or saltwater up to the tinned lead connector.
We also offer a unique Mini Lab Grade DO probe that is perfect for small spaces, giving you the same quality readings as the Lab Grade DO probe, however, as it is smaller, it may need to be refilled with electrolyte more frequently than regular DO probes.
Remember to always check the manufacturer’s manual on calibration before use.
What Affects Dissolved Oxygen In Water?
When measuring DO, it is important to take into consideration factors that can affect the measurements. These include the following:
Dissolved oxygen probes work by measuring the amount of oxygen that diffuses across a membrane into the sensor. Once oxygen is inside the sensor, a chemical reduction reaction occurs, producing an electrical signal. This signal is read by the DO probe and is displayed on a meter.
If you would like to learn more about other water quality measurements, characteristics, or what DO probes we have to offer, do not hesitate to contact our world-class team at Atlas Scientific.
There are many different types of bioreactors including bubble column bioreactors, continuous stirred tank bioreactors, fluidized bed bioreactors, packed bed bioreactors, and photobioreactors. The most common bioreactors are the stirred tank, airlift, and fixed-bed types. Bioreactors are essential tools in biotechnology and have revolutionized research in various fields. They are most commonly used in the
pH refers to the level of acidity or alkalinity in meat, and it plays a crucial role in determining the quality of the meat you eat. A proper pH level ensures that the meat is fresh, tender, and juicy. It also helps in preserving the meat for a longer period of time. When the pH